Disruptions in gastrointestinal microbiome and gut function can result in both localized and widespread health issues. The GI360™ Profile is an innovative, comprehensive, and clinically applicable stool profile that can aid in detecting underlying causes of symptoms, improving treatment strategies and patient outcomes. The profile includes dysbiosis, abundance and diversity assessments of the gut microbiome based on 45 peer-reviewed analytes shown to contribute to dysbiosis and other chronic disease states, GI pathogens via multiplex PCR parasitology via microscopy, bacterial microbiology with susceptibilities (prescriptive and natural agents) and stool chemistries.